Review



emd 50336 cryo em structural model  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    ATCC emd 50336 cryo em structural model
    Emd 50336 Cryo Em Structural Model, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/emd+50336+cryo+em+structural+model/Hexamita+pusilla+Klebs/pm41321628-210-194-221
    Average 90 stars, based on 2 article reviews
    emd 50336 cryo em structural model - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Development of DARPin T cell engagers for specific targeting of tumor-associated HLA/peptide complexes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Live Dead stain aqua (for FACS) Thermo L34957 Live/Dead stain FITC (for FACS) Thermo L23101 APC mouse anti-human IFN-g (for FACS) BD 554702; RRID:AB_398580 Pacific Blue mouse anti-human CD8 (for FACS) BD 558207; RRID:AB_397058 CD8 Alexa 488 (for FACS) BD 557696; RRID:AB_396805 CD25 PerCP Cy5.5 (for FACS) ebio 45-0259-42; RRID:AB_11043556 CD69 Pe-Cy7 BD 560712; RRID:AB_1727509 CD4 efluor 450 ebio 48-0048-42; RRID:AB_2016674 CD3 PE ebio 12-0037-42; RRID:AB_1272078 Penta-His AF488 Qiagen 35310; RRID:AB_3083465 anti-6His-D2 (HTRF assay) Cisbio 61HISDLB; RRID:AB_2884027 1:400 anti-strep-Tb (HTRF assay) Cisbio 610SATLB RGS-His antibody (SPR) Qiagen 34650; RRID:AB_2687898 Bacterial and virus strains E. coli BL21 (DE3) Novagen 69450–3 E. coli XL1-Blue Stratagene 200228 Chemicals, peptides, and recombinant proteins Peptide NY-ESO1 157-165 (9V): SLLMWITQV Genscript Custom synthesis Peptide NY-ESO1 157-165 (9VAA): SLLAAITQV Genscript Custom synthesis Peptide EBNA-1 371 FMVFLQTHI Genscript Custom synthesis Peptides identified as potentially crossreactive (listed in Table S2) Genscript Custom synthesis Bacterial lysis buffer B-PERII Thermo 78260 CD8 + T cell Isolation Kit, human Milteny Biotec 130-096-495 human IL-2 Immunotools 11340025 Morpheus screen (protein crystallization) Molecular Dimensions MD1-46 BS(PEG)5 (cross-linking) Thermo Fisher A35396 Deposited data Cryo-EM map of the ternary DARPin NY_1/ HLA-A0201/NY-ESO1 complex. https://www.ebi.ac.uk/emdb/EMD-50336 EMD-50336 Cryo-EM structural model of the ternary DARPin NY_1/HLA-A0201/NY-ESO1 complex. https://www.rcsb.org/structure/9FE1 9FE1 Crystal structure of DARPin NY_1 https://www.rcsb.org/structure/9EPA 9EPA Experimental models: Cell lines T2 (Tumor cell line) ATCC CRL-1992 MCF-7 (Tumor cell line) ATCC HTB-22 U266B1 (Tumor cell line) ATCC TIB-196 IM9 (Tumor cell line) ATCC CCL-159 Colo-205 (Tumor cell line) ECACC 87061208 HCT116 (Tumor cell line) ATCC CCL-247 NCI-H1755 (Tumor cell line) ATCC CRL-5892 NCI-H1703 (Tumor cell line) ATCC CRL-5889 (Continued on next page) e1 iScience 28, 113926, December 19, 2025 ..

    FACS:

    Article Title: Development of DARPin T cell engagers for specific targeting of tumor-associated HLA/peptide complexes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Live Dead stain aqua (for FACS) Thermo L34957 Live/Dead stain FITC (for FACS) Thermo L23101 APC mouse anti-human IFN-g (for FACS) BD 554702; RRID:AB_398580 Pacific Blue mouse anti-human CD8 (for FACS) BD 558207; RRID:AB_397058 CD8 Alexa 488 (for FACS) BD 557696; RRID:AB_396805 CD25 PerCP Cy5.5 (for FACS) ebio 45-0259-42; RRID:AB_11043556 CD69 Pe-Cy7 BD 560712; RRID:AB_1727509 CD4 efluor 450 ebio 48-0048-42; RRID:AB_2016674 CD3 PE ebio 12-0037-42; RRID:AB_1272078 Penta-His AF488 Qiagen 35310; RRID:AB_3083465 anti-6His-D2 (HTRF assay) Cisbio 61HISDLB; RRID:AB_2884027 1:400 anti-strep-Tb (HTRF assay) Cisbio 610SATLB RGS-His antibody (SPR) Qiagen 34650; RRID:AB_2687898 Bacterial and virus strains E. coli BL21 (DE3) Novagen 69450–3 E. coli XL1-Blue Stratagene 200228 Chemicals, peptides, and recombinant proteins Peptide NY-ESO1 157-165 (9V): SLLMWITQV Genscript Custom synthesis Peptide NY-ESO1 157-165 (9VAA): SLLAAITQV Genscript Custom synthesis Peptide EBNA-1 371 FMVFLQTHI Genscript Custom synthesis Peptides identified as potentially crossreactive (listed in Table S2) Genscript Custom synthesis Bacterial lysis buffer B-PERII Thermo 78260 CD8 + T cell Isolation Kit, human Milteny Biotec 130-096-495 human IL-2 Immunotools 11340025 Morpheus screen (protein crystallization) Molecular Dimensions MD1-46 BS(PEG)5 (cross-linking) Thermo Fisher A35396 Deposited data Cryo-EM map of the ternary DARPin NY_1/ HLA-A0201/NY-ESO1 complex. https://www.ebi.ac.uk/emdb/EMD-50336 EMD-50336 Cryo-EM structural model of the ternary DARPin NY_1/HLA-A0201/NY-ESO1 complex. https://www.rcsb.org/structure/9FE1 9FE1 Crystal structure of DARPin NY_1 https://www.rcsb.org/structure/9EPA 9EPA Experimental models: Cell lines T2 (Tumor cell line) ATCC CRL-1992 MCF-7 (Tumor cell line) ATCC HTB-22 U266B1 (Tumor cell line) ATCC TIB-196 IM9 (Tumor cell line) ATCC CCL-159 Colo-205 (Tumor cell line) ECACC 87061208 HCT116 (Tumor cell line) ATCC CCL-247 NCI-H1755 (Tumor cell line) ATCC CRL-5892 NCI-H1703 (Tumor cell line) ATCC CRL-5889 (Continued on next page) e1 iScience 28, 113926, December 19, 2025 ..

    HTRF Assay:

    Article Title: Development of DARPin T cell engagers for specific targeting of tumor-associated HLA/peptide complexes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Live Dead stain aqua (for FACS) Thermo L34957 Live/Dead stain FITC (for FACS) Thermo L23101 APC mouse anti-human IFN-g (for FACS) BD 554702; RRID:AB_398580 Pacific Blue mouse anti-human CD8 (for FACS) BD 558207; RRID:AB_397058 CD8 Alexa 488 (for FACS) BD 557696; RRID:AB_396805 CD25 PerCP Cy5.5 (for FACS) ebio 45-0259-42; RRID:AB_11043556 CD69 Pe-Cy7 BD 560712; RRID:AB_1727509 CD4 efluor 450 ebio 48-0048-42; RRID:AB_2016674 CD3 PE ebio 12-0037-42; RRID:AB_1272078 Penta-His AF488 Qiagen 35310; RRID:AB_3083465 anti-6His-D2 (HTRF assay) Cisbio 61HISDLB; RRID:AB_2884027 1:400 anti-strep-Tb (HTRF assay) Cisbio 610SATLB RGS-His antibody (SPR) Qiagen 34650; RRID:AB_2687898 Bacterial and virus strains E. coli BL21 (DE3) Novagen 69450–3 E. coli XL1-Blue Stratagene 200228 Chemicals, peptides, and recombinant proteins Peptide NY-ESO1 157-165 (9V): SLLMWITQV Genscript Custom synthesis Peptide NY-ESO1 157-165 (9VAA): SLLAAITQV Genscript Custom synthesis Peptide EBNA-1 371 FMVFLQTHI Genscript Custom synthesis Peptides identified as potentially crossreactive (listed in Table S2) Genscript Custom synthesis Bacterial lysis buffer B-PERII Thermo 78260 CD8 + T cell Isolation Kit, human Milteny Biotec 130-096-495 human IL-2 Immunotools 11340025 Morpheus screen (protein crystallization) Molecular Dimensions MD1-46 BS(PEG)5 (cross-linking) Thermo Fisher A35396 Deposited data Cryo-EM map of the ternary DARPin NY_1/ HLA-A0201/NY-ESO1 complex. https://www.ebi.ac.uk/emdb/EMD-50336 EMD-50336 Cryo-EM structural model of the ternary DARPin NY_1/HLA-A0201/NY-ESO1 complex. https://www.rcsb.org/structure/9FE1 9FE1 Crystal structure of DARPin NY_1 https://www.rcsb.org/structure/9EPA 9EPA Experimental models: Cell lines T2 (Tumor cell line) ATCC CRL-1992 MCF-7 (Tumor cell line) ATCC HTB-22 U266B1 (Tumor cell line) ATCC TIB-196 IM9 (Tumor cell line) ATCC CCL-159 Colo-205 (Tumor cell line) ECACC 87061208 HCT116 (Tumor cell line) ATCC CCL-247 NCI-H1755 (Tumor cell line) ATCC CRL-5892 NCI-H1703 (Tumor cell line) ATCC CRL-5889 (Continued on next page) e1 iScience 28, 113926, December 19, 2025 ..

    SPR Assay:

    Article Title: Development of DARPin T cell engagers for specific targeting of tumor-associated HLA/peptide complexes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Live Dead stain aqua (for FACS) Thermo L34957 Live/Dead stain FITC (for FACS) Thermo L23101 APC mouse anti-human IFN-g (for FACS) BD 554702; RRID:AB_398580 Pacific Blue mouse anti-human CD8 (for FACS) BD 558207; RRID:AB_397058 CD8 Alexa 488 (for FACS) BD 557696; RRID:AB_396805 CD25 PerCP Cy5.5 (for FACS) ebio 45-0259-42; RRID:AB_11043556 CD69 Pe-Cy7 BD 560712; RRID:AB_1727509 CD4 efluor 450 ebio 48-0048-42; RRID:AB_2016674 CD3 PE ebio 12-0037-42; RRID:AB_1272078 Penta-His AF488 Qiagen 35310; RRID:AB_3083465 anti-6His-D2 (HTRF assay) Cisbio 61HISDLB; RRID:AB_2884027 1:400 anti-strep-Tb (HTRF assay) Cisbio 610SATLB RGS-His antibody (SPR) Qiagen 34650; RRID:AB_2687898 Bacterial and virus strains E. coli BL21 (DE3) Novagen 69450–3 E. coli XL1-Blue Stratagene 200228 Chemicals, peptides, and recombinant proteins Peptide NY-ESO1 157-165 (9V): SLLMWITQV Genscript Custom synthesis Peptide NY-ESO1 157-165 (9VAA): SLLAAITQV Genscript Custom synthesis Peptide EBNA-1 371 FMVFLQTHI Genscript Custom synthesis Peptides identified as potentially crossreactive (listed in Table S2) Genscript Custom synthesis Bacterial lysis buffer B-PERII Thermo 78260 CD8 + T cell Isolation Kit, human Milteny Biotec 130-096-495 human IL-2 Immunotools 11340025 Morpheus screen (protein crystallization) Molecular Dimensions MD1-46 BS(PEG)5 (cross-linking) Thermo Fisher A35396 Deposited data Cryo-EM map of the ternary DARPin NY_1/ HLA-A0201/NY-ESO1 complex. https://www.ebi.ac.uk/emdb/EMD-50336 EMD-50336 Cryo-EM structural model of the ternary DARPin NY_1/HLA-A0201/NY-ESO1 complex. https://www.rcsb.org/structure/9FE1 9FE1 Crystal structure of DARPin NY_1 https://www.rcsb.org/structure/9EPA 9EPA Experimental models: Cell lines T2 (Tumor cell line) ATCC CRL-1992 MCF-7 (Tumor cell line) ATCC HTB-22 U266B1 (Tumor cell line) ATCC TIB-196 IM9 (Tumor cell line) ATCC CCL-159 Colo-205 (Tumor cell line) ECACC 87061208 HCT116 (Tumor cell line) ATCC CCL-247 NCI-H1755 (Tumor cell line) ATCC CRL-5892 NCI-H1703 (Tumor cell line) ATCC CRL-5889 (Continued on next page) e1 iScience 28, 113926, December 19, 2025 ..

    Virus:

    Article Title: Development of DARPin T cell engagers for specific targeting of tumor-associated HLA/peptide complexes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Live Dead stain aqua (for FACS) Thermo L34957 Live/Dead stain FITC (for FACS) Thermo L23101 APC mouse anti-human IFN-g (for FACS) BD 554702; RRID:AB_398580 Pacific Blue mouse anti-human CD8 (for FACS) BD 558207; RRID:AB_397058 CD8 Alexa 488 (for FACS) BD 557696; RRID:AB_396805 CD25 PerCP Cy5.5 (for FACS) ebio 45-0259-42; RRID:AB_11043556 CD69 Pe-Cy7 BD 560712; RRID:AB_1727509 CD4 efluor 450 ebio 48-0048-42; RRID:AB_2016674 CD3 PE ebio 12-0037-42; RRID:AB_1272078 Penta-His AF488 Qiagen 35310; RRID:AB_3083465 anti-6His-D2 (HTRF assay) Cisbio 61HISDLB; RRID:AB_2884027 1:400 anti-strep-Tb (HTRF assay) Cisbio 610SATLB RGS-His antibody (SPR) Qiagen 34650; RRID:AB_2687898 Bacterial and virus strains E. coli BL21 (DE3) Novagen 69450–3 E. coli XL1-Blue Stratagene 200228 Chemicals, peptides, and recombinant proteins Peptide NY-ESO1 157-165 (9V): SLLMWITQV Genscript Custom synthesis Peptide NY-ESO1 157-165 (9VAA): SLLAAITQV Genscript Custom synthesis Peptide EBNA-1 371 FMVFLQTHI Genscript Custom synthesis Peptides identified as potentially crossreactive (listed in Table S2) Genscript Custom synthesis Bacterial lysis buffer B-PERII Thermo 78260 CD8 + T cell Isolation Kit, human Milteny Biotec 130-096-495 human IL-2 Immunotools 11340025 Morpheus screen (protein crystallization) Molecular Dimensions MD1-46 BS(PEG)5 (cross-linking) Thermo Fisher A35396 Deposited data Cryo-EM map of the ternary DARPin NY_1/ HLA-A0201/NY-ESO1 complex. https://www.ebi.ac.uk/emdb/EMD-50336 EMD-50336 Cryo-EM structural model of the ternary DARPin NY_1/HLA-A0201/NY-ESO1 complex. https://www.rcsb.org/structure/9FE1 9FE1 Crystal structure of DARPin NY_1 https://www.rcsb.org/structure/9EPA 9EPA Experimental models: Cell lines T2 (Tumor cell line) ATCC CRL-1992 MCF-7 (Tumor cell line) ATCC HTB-22 U266B1 (Tumor cell line) ATCC TIB-196 IM9 (Tumor cell line) ATCC CCL-159 Colo-205 (Tumor cell line) ECACC 87061208 HCT116 (Tumor cell line) ATCC CCL-247 NCI-H1755 (Tumor cell line) ATCC CRL-5892 NCI-H1703 (Tumor cell line) ATCC CRL-5889 (Continued on next page) e1 iScience 28, 113926, December 19, 2025 ..

    Recombinant:

    Article Title: Development of DARPin T cell engagers for specific targeting of tumor-associated HLA/peptide complexes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Live Dead stain aqua (for FACS) Thermo L34957 Live/Dead stain FITC (for FACS) Thermo L23101 APC mouse anti-human IFN-g (for FACS) BD 554702; RRID:AB_398580 Pacific Blue mouse anti-human CD8 (for FACS) BD 558207; RRID:AB_397058 CD8 Alexa 488 (for FACS) BD 557696; RRID:AB_396805 CD25 PerCP Cy5.5 (for FACS) ebio 45-0259-42; RRID:AB_11043556 CD69 Pe-Cy7 BD 560712; RRID:AB_1727509 CD4 efluor 450 ebio 48-0048-42; RRID:AB_2016674 CD3 PE ebio 12-0037-42; RRID:AB_1272078 Penta-His AF488 Qiagen 35310; RRID:AB_3083465 anti-6His-D2 (HTRF assay) Cisbio 61HISDLB; RRID:AB_2884027 1:400 anti-strep-Tb (HTRF assay) Cisbio 610SATLB RGS-His antibody (SPR) Qiagen 34650; RRID:AB_2687898 Bacterial and virus strains E. coli BL21 (DE3) Novagen 69450–3 E. coli XL1-Blue Stratagene 200228 Chemicals, peptides, and recombinant proteins Peptide NY-ESO1 157-165 (9V): SLLMWITQV Genscript Custom synthesis Peptide NY-ESO1 157-165 (9VAA): SLLAAITQV Genscript Custom synthesis Peptide EBNA-1 371 FMVFLQTHI Genscript Custom synthesis Peptides identified as potentially crossreactive (listed in Table S2) Genscript Custom synthesis Bacterial lysis buffer B-PERII Thermo 78260 CD8 + T cell Isolation Kit, human Milteny Biotec 130-096-495 human IL-2 Immunotools 11340025 Morpheus screen (protein crystallization) Molecular Dimensions MD1-46 BS(PEG)5 (cross-linking) Thermo Fisher A35396 Deposited data Cryo-EM map of the ternary DARPin NY_1/ HLA-A0201/NY-ESO1 complex. https://www.ebi.ac.uk/emdb/EMD-50336 EMD-50336 Cryo-EM structural model of the ternary DARPin NY_1/HLA-A0201/NY-ESO1 complex. https://www.rcsb.org/structure/9FE1 9FE1 Crystal structure of DARPin NY_1 https://www.rcsb.org/structure/9EPA 9EPA Experimental models: Cell lines T2 (Tumor cell line) ATCC CRL-1992 MCF-7 (Tumor cell line) ATCC HTB-22 U266B1 (Tumor cell line) ATCC TIB-196 IM9 (Tumor cell line) ATCC CCL-159 Colo-205 (Tumor cell line) ECACC 87061208 HCT116 (Tumor cell line) ATCC CCL-247 NCI-H1755 (Tumor cell line) ATCC CRL-5892 NCI-H1703 (Tumor cell line) ATCC CRL-5889 (Continued on next page) e1 iScience 28, 113926, December 19, 2025 ..

    Lysis:

    Article Title: Development of DARPin T cell engagers for specific targeting of tumor-associated HLA/peptide complexes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Live Dead stain aqua (for FACS) Thermo L34957 Live/Dead stain FITC (for FACS) Thermo L23101 APC mouse anti-human IFN-g (for FACS) BD 554702; RRID:AB_398580 Pacific Blue mouse anti-human CD8 (for FACS) BD 558207; RRID:AB_397058 CD8 Alexa 488 (for FACS) BD 557696; RRID:AB_396805 CD25 PerCP Cy5.5 (for FACS) ebio 45-0259-42; RRID:AB_11043556 CD69 Pe-Cy7 BD 560712; RRID:AB_1727509 CD4 efluor 450 ebio 48-0048-42; RRID:AB_2016674 CD3 PE ebio 12-0037-42; RRID:AB_1272078 Penta-His AF488 Qiagen 35310; RRID:AB_3083465 anti-6His-D2 (HTRF assay) Cisbio 61HISDLB; RRID:AB_2884027 1:400 anti-strep-Tb (HTRF assay) Cisbio 610SATLB RGS-His antibody (SPR) Qiagen 34650; RRID:AB_2687898 Bacterial and virus strains E. coli BL21 (DE3) Novagen 69450–3 E. coli XL1-Blue Stratagene 200228 Chemicals, peptides, and recombinant proteins Peptide NY-ESO1 157-165 (9V): SLLMWITQV Genscript Custom synthesis Peptide NY-ESO1 157-165 (9VAA): SLLAAITQV Genscript Custom synthesis Peptide EBNA-1 371 FMVFLQTHI Genscript Custom synthesis Peptides identified as potentially crossreactive (listed in Table S2) Genscript Custom synthesis Bacterial lysis buffer B-PERII Thermo 78260 CD8 + T cell Isolation Kit, human Milteny Biotec 130-096-495 human IL-2 Immunotools 11340025 Morpheus screen (protein crystallization) Molecular Dimensions MD1-46 BS(PEG)5 (cross-linking) Thermo Fisher A35396 Deposited data Cryo-EM map of the ternary DARPin NY_1/ HLA-A0201/NY-ESO1 complex. https://www.ebi.ac.uk/emdb/EMD-50336 EMD-50336 Cryo-EM structural model of the ternary DARPin NY_1/HLA-A0201/NY-ESO1 complex. https://www.rcsb.org/structure/9FE1 9FE1 Crystal structure of DARPin NY_1 https://www.rcsb.org/structure/9EPA 9EPA Experimental models: Cell lines T2 (Tumor cell line) ATCC CRL-1992 MCF-7 (Tumor cell line) ATCC HTB-22 U266B1 (Tumor cell line) ATCC TIB-196 IM9 (Tumor cell line) ATCC CCL-159 Colo-205 (Tumor cell line) ECACC 87061208 HCT116 (Tumor cell line) ATCC CCL-247 NCI-H1755 (Tumor cell line) ATCC CRL-5892 NCI-H1703 (Tumor cell line) ATCC CRL-5889 (Continued on next page) e1 iScience 28, 113926, December 19, 2025 ..

    Cell Isolation:

    Article Title: Development of DARPin T cell engagers for specific targeting of tumor-associated HLA/peptide complexes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Live Dead stain aqua (for FACS) Thermo L34957 Live/Dead stain FITC (for FACS) Thermo L23101 APC mouse anti-human IFN-g (for FACS) BD 554702; RRID:AB_398580 Pacific Blue mouse anti-human CD8 (for FACS) BD 558207; RRID:AB_397058 CD8 Alexa 488 (for FACS) BD 557696; RRID:AB_396805 CD25 PerCP Cy5.5 (for FACS) ebio 45-0259-42; RRID:AB_11043556 CD69 Pe-Cy7 BD 560712; RRID:AB_1727509 CD4 efluor 450 ebio 48-0048-42; RRID:AB_2016674 CD3 PE ebio 12-0037-42; RRID:AB_1272078 Penta-His AF488 Qiagen 35310; RRID:AB_3083465 anti-6His-D2 (HTRF assay) Cisbio 61HISDLB; RRID:AB_2884027 1:400 anti-strep-Tb (HTRF assay) Cisbio 610SATLB RGS-His antibody (SPR) Qiagen 34650; RRID:AB_2687898 Bacterial and virus strains E. coli BL21 (DE3) Novagen 69450–3 E. coli XL1-Blue Stratagene 200228 Chemicals, peptides, and recombinant proteins Peptide NY-ESO1 157-165 (9V): SLLMWITQV Genscript Custom synthesis Peptide NY-ESO1 157-165 (9VAA): SLLAAITQV Genscript Custom synthesis Peptide EBNA-1 371 FMVFLQTHI Genscript Custom synthesis Peptides identified as potentially crossreactive (listed in Table S2) Genscript Custom synthesis Bacterial lysis buffer B-PERII Thermo 78260 CD8 + T cell Isolation Kit, human Milteny Biotec 130-096-495 human IL-2 Immunotools 11340025 Morpheus screen (protein crystallization) Molecular Dimensions MD1-46 BS(PEG)5 (cross-linking) Thermo Fisher A35396 Deposited data Cryo-EM map of the ternary DARPin NY_1/ HLA-A0201/NY-ESO1 complex. https://www.ebi.ac.uk/emdb/EMD-50336 EMD-50336 Cryo-EM structural model of the ternary DARPin NY_1/HLA-A0201/NY-ESO1 complex. https://www.rcsb.org/structure/9FE1 9FE1 Crystal structure of DARPin NY_1 https://www.rcsb.org/structure/9EPA 9EPA Experimental models: Cell lines T2 (Tumor cell line) ATCC CRL-1992 MCF-7 (Tumor cell line) ATCC HTB-22 U266B1 (Tumor cell line) ATCC TIB-196 IM9 (Tumor cell line) ATCC CCL-159 Colo-205 (Tumor cell line) ECACC 87061208 HCT116 (Tumor cell line) ATCC CCL-247 NCI-H1755 (Tumor cell line) ATCC CRL-5892 NCI-H1703 (Tumor cell line) ATCC CRL-5889 (Continued on next page) e1 iScience 28, 113926, December 19, 2025 ..

    Crystallization Assay:

    Article Title: Development of DARPin T cell engagers for specific targeting of tumor-associated HLA/peptide complexes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Live Dead stain aqua (for FACS) Thermo L34957 Live/Dead stain FITC (for FACS) Thermo L23101 APC mouse anti-human IFN-g (for FACS) BD 554702; RRID:AB_398580 Pacific Blue mouse anti-human CD8 (for FACS) BD 558207; RRID:AB_397058 CD8 Alexa 488 (for FACS) BD 557696; RRID:AB_396805 CD25 PerCP Cy5.5 (for FACS) ebio 45-0259-42; RRID:AB_11043556 CD69 Pe-Cy7 BD 560712; RRID:AB_1727509 CD4 efluor 450 ebio 48-0048-42; RRID:AB_2016674 CD3 PE ebio 12-0037-42; RRID:AB_1272078 Penta-His AF488 Qiagen 35310; RRID:AB_3083465 anti-6His-D2 (HTRF assay) Cisbio 61HISDLB; RRID:AB_2884027 1:400 anti-strep-Tb (HTRF assay) Cisbio 610SATLB RGS-His antibody (SPR) Qiagen 34650; RRID:AB_2687898 Bacterial and virus strains E. coli BL21 (DE3) Novagen 69450–3 E. coli XL1-Blue Stratagene 200228 Chemicals, peptides, and recombinant proteins Peptide NY-ESO1 157-165 (9V): SLLMWITQV Genscript Custom synthesis Peptide NY-ESO1 157-165 (9VAA): SLLAAITQV Genscript Custom synthesis Peptide EBNA-1 371 FMVFLQTHI Genscript Custom synthesis Peptides identified as potentially crossreactive (listed in Table S2) Genscript Custom synthesis Bacterial lysis buffer B-PERII Thermo 78260 CD8 + T cell Isolation Kit, human Milteny Biotec 130-096-495 human IL-2 Immunotools 11340025 Morpheus screen (protein crystallization) Molecular Dimensions MD1-46 BS(PEG)5 (cross-linking) Thermo Fisher A35396 Deposited data Cryo-EM map of the ternary DARPin NY_1/ HLA-A0201/NY-ESO1 complex. https://www.ebi.ac.uk/emdb/EMD-50336 EMD-50336 Cryo-EM structural model of the ternary DARPin NY_1/HLA-A0201/NY-ESO1 complex. https://www.rcsb.org/structure/9FE1 9FE1 Crystal structure of DARPin NY_1 https://www.rcsb.org/structure/9EPA 9EPA Experimental models: Cell lines T2 (Tumor cell line) ATCC CRL-1992 MCF-7 (Tumor cell line) ATCC HTB-22 U266B1 (Tumor cell line) ATCC TIB-196 IM9 (Tumor cell line) ATCC CCL-159 Colo-205 (Tumor cell line) ECACC 87061208 HCT116 (Tumor cell line) ATCC CCL-247 NCI-H1755 (Tumor cell line) ATCC CRL-5892 NCI-H1703 (Tumor cell line) ATCC CRL-5889 (Continued on next page) e1 iScience 28, 113926, December 19, 2025 ..

    Cryo-EM Sample Prep:

    Article Title: Development of DARPin T cell engagers for specific targeting of tumor-associated HLA/peptide complexes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Live Dead stain aqua (for FACS) Thermo L34957 Live/Dead stain FITC (for FACS) Thermo L23101 APC mouse anti-human IFN-g (for FACS) BD 554702; RRID:AB_398580 Pacific Blue mouse anti-human CD8 (for FACS) BD 558207; RRID:AB_397058 CD8 Alexa 488 (for FACS) BD 557696; RRID:AB_396805 CD25 PerCP Cy5.5 (for FACS) ebio 45-0259-42; RRID:AB_11043556 CD69 Pe-Cy7 BD 560712; RRID:AB_1727509 CD4 efluor 450 ebio 48-0048-42; RRID:AB_2016674 CD3 PE ebio 12-0037-42; RRID:AB_1272078 Penta-His AF488 Qiagen 35310; RRID:AB_3083465 anti-6His-D2 (HTRF assay) Cisbio 61HISDLB; RRID:AB_2884027 1:400 anti-strep-Tb (HTRF assay) Cisbio 610SATLB RGS-His antibody (SPR) Qiagen 34650; RRID:AB_2687898 Bacterial and virus strains E. coli BL21 (DE3) Novagen 69450–3 E. coli XL1-Blue Stratagene 200228 Chemicals, peptides, and recombinant proteins Peptide NY-ESO1 157-165 (9V): SLLMWITQV Genscript Custom synthesis Peptide NY-ESO1 157-165 (9VAA): SLLAAITQV Genscript Custom synthesis Peptide EBNA-1 371 FMVFLQTHI Genscript Custom synthesis Peptides identified as potentially crossreactive (listed in Table S2) Genscript Custom synthesis Bacterial lysis buffer B-PERII Thermo 78260 CD8 + T cell Isolation Kit, human Milteny Biotec 130-096-495 human IL-2 Immunotools 11340025 Morpheus screen (protein crystallization) Molecular Dimensions MD1-46 BS(PEG)5 (cross-linking) Thermo Fisher A35396 Deposited data Cryo-EM map of the ternary DARPin NY_1/ HLA-A0201/NY-ESO1 complex. https://www.ebi.ac.uk/emdb/EMD-50336 EMD-50336 Cryo-EM structural model of the ternary DARPin NY_1/HLA-A0201/NY-ESO1 complex. https://www.rcsb.org/structure/9FE1 9FE1 Crystal structure of DARPin NY_1 https://www.rcsb.org/structure/9EPA 9EPA Experimental models: Cell lines T2 (Tumor cell line) ATCC CRL-1992 MCF-7 (Tumor cell line) ATCC HTB-22 U266B1 (Tumor cell line) ATCC TIB-196 IM9 (Tumor cell line) ATCC CCL-159 Colo-205 (Tumor cell line) ECACC 87061208 HCT116 (Tumor cell line) ATCC CCL-247 NCI-H1755 (Tumor cell line) ATCC CRL-5892 NCI-H1703 (Tumor cell line) ATCC CRL-5889 (Continued on next page) e1 iScience 28, 113926, December 19, 2025 ..



    Similar Products

    emd 50336 cryo em structural model  (ATCC)
    90
    ATCC emd 50336 cryo em structural model
    Emd 50336 Cryo Em Structural Model, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/emd+50336+cryo+em+structural+model/Hexamita+pusilla+Klebs/pm41321628-210-194-221
    Average 90 stars, based on 1 article reviews
    emd 50336 cryo em structural model - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results